Available at: https://digitalcommons.calpoly.edu/theses/3185
Date of Award
12-2025
Degree Name
MS in Biomedical Engineering
Department/Program
Biomedical Engineering
College
College of Engineering
Advisor
Christopher Heylman
Advisor Department
Biomedical Engineering
Advisor College
College of Engineering
Abstract
This work demonstrates the feasibility of a novel microfluidic tumor model that reproduces physiologically relevant nutrient gradients and endothelium–tumor interactions, validating its potential as a preclinical test platform. Finite element simulations of glutamine diffusion and fluid dynamics aligned with experimental tracer studies, and co-cultures supported directed endothelial migration toward tumor spheroids, confirming the device’s ability to mimic in vivo–like behavior.
Current preclinical models often fail to capture the complexity of the tumor microenvironment (TME), limiting their predictive value for therapeutic testing. Nutrient availability and angiogenic signaling are critical drivers of tumor progression, yet they are poorly represented in traditional in vitro systems.
To address this gap, a microfluidic device incorporating a colorectal cancer tumor compartment with a central endothelial lined tube was designed. Computational models of transport were developed and compared to experimental measurements, and the device’s biological relevance was assessed with tumor–endothelial co-cultures. The findings highlight the device’s promise of bridging the gap between conventional in vitro assays and in vivo studies, ultimately advancing the development of more reliable platforms for therapeutic evaluation.