Available at: https://digitalcommons.calpoly.edu/theses/2973
Date of Award
3-2024
Degree Name
MS in Biomedical Engineering
Department/Program
Biomedical Engineering
College
College of Engineering
Advisor
Christopher Heylman
Advisor Department
Biomedical Engineering
Advisor College
College of Engineering
Abstract
In order to perform more robust and physiologically relevant research, the Microphysiological Systems laboratory required a method of culturing cells where the level of oxygen could be adjusted. To fulfill this need, I designed and manufactured a hypoxic cell culture incubator. I employed a Quality Function Deployment to understand the customer requirements and convert them into engineering specifications. Based on these specifications, I created various conceptual designs. I evaluated the conceptual designs as compared to existing products and decided to move forward with the control system separated from a small, standard chamber. I developed the circuitry schematics for the temperature control loop, incorporating three heating pads and a temperature sensor, and the gas concentration control loop, incorporating three solenoids and two gas concentration sensors for oxygen and carbon dioxide. I designed the chamber in SolidWorks and 3D printed it. Finally, I performed vigorous validation testing of the device’s ability to control the temperature, the concentration of carbon dioxide, and the concentration of oxygen. The incubator was able to maintain the temperature within 0.5 degrees Celsius of a setpoint, the concentration of carbon dioxide within 0.24% of a setpoint, and the concentration of oxygen within 0.05% of a setpoint. These results surpassed the determined requirements.
