DOI: https://doi.org/10.15368/theses.2020.166
Available at: https://digitalcommons.calpoly.edu/theses/2244
Date of Award
12-2020
Degree Name
MS in Engineering - Biochemical Engineering
Department/Program
Biomedical and General Engineering
College
College of Engineering
Advisor
Benjamin Hawkins
Advisor Department
Biomedical and General Engineering
Advisor College
College of Engineering
Abstract
The goal of this study is to design a protocol for the adherent cell culture within a novel microfluidic device. Microscale cell culture protocols were developed for loading cells using poly-L-lysine to enhance adherent cell culture of murine derived NIH 3T3 fibroblasts. This work sought to develop a method for adherent microculture by examining various sterilization, surface treatment, and seeding techniques. Using a vacuum suction loading technique, air plasma treatment and a poly-L-lysine surface treatment adherent cell culture was observed within the device. The work presented here is part of a collaborative effort that aims to develop protocols for the electrical and optical characterization of cell culture within a novel microfluidic device.