DOI: https://doi.org/10.15368/theses.2018.146
Available at: https://digitalcommons.calpoly.edu/theses/1950
Date of Award
9-2018
Degree Name
MS in Biomedical Engineering
Department/Program
Biomedical and General Engineering
Advisor
Benjamin Hawkins
Abstract
The goal of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created in PDMS via soft lithography. The device measured ethanol impedance initially to show that values remain consistent over time. Impedance values of water and 1% wt. saltwater were compared to show that the device is able to detect changes in impedance, with up to a 60% reduction in electrical impedance in saltwater. Cells were introduced into the device, where changes in impedance were seen across multiple frequencies, indicating that the device is capable of detecting the presence of biologic elements within a system. Cell measurements were performed using NIH-3T3 fibroblasts.
Included in
Biological Engineering Commons, Biomechanics and Biotransport Commons, Biomedical Commons, Biomedical Devices and Instrumentation Commons, Biotechnology Commons