Title
Analyzing Environmental Microbes for Genomic Regions Promoting Ionic Liquid Tolerance in E. coli
Recommended Citation
August 1, 2012.
Abstract
Ionic liquids (ILs) are promising as solvents to increase the efficiency of biofuel production; however, ILs are toxic to microbes used in the fermentation of liquid fuels. To engineer IL resistant biofuel hosts, environmental bacteria were screened for tolerance, and these were used to create gene libraries to test in E. coli. Future characterization of these libraries using molecular techniques will be used to identify genes that contribute IL-tolerance to transformed microbes.
Disciplines
Biochemistry | Biology | Biotechnology | Laboratory and Basic Science Research | Life Sciences | Molecular Biology | Other Microbiology
Mentor
Michael P Thelen
Lab site
Lawrence Livermore National Laboratory (LLNL)
Funding Acknowledgement
This material is based upon work supported by the S.D. Bechtel, Jr. Foundation and by the National Science Foundation under Grant No. 0952013. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the S.D. Bechtel, Jr. Foundation or the National Science Foundation. This project has also been made possible with support of the National Marine Sanctuary Foundation. The STAR program is administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESaME) on behalf of the California State University (CSU).
Included in
Biochemistry Commons, Biology Commons, Biotechnology Commons, Laboratory and Basic Science Research Commons, Molecular Biology Commons, Other Microbiology Commons
URL: https://digitalcommons.calpoly.edu/star/124