To find a gene that confers ionic liquid resistance in bacteria, environmental samples of bacteria were taken from a high salt environment in the FarallonIslands. The bacteria were grown on different media that contained either 1‐Ethyl‐3‐methylimidazolium chloride (EMiM‐Cl) or 1‐Ethyl‐3‐ methylimidazolium acetate (EMiM‐Ac) to determine which bacteria should be used to create a genomic library to screen for genes conferring ionic liquid resistance. Bacterial isolates IL 210, IL 15, CX32 AND CX 38 grow on media containing EMiM‐Cl as well as media containing EMiM‐Ac and are recommended for the creation of a gene library.


Michael Thelen

Lab site

Lawrence Livermore National Laboratory (LLNL)

Funding Acknowledgement

This material is based upon work supported by the S.D. Bechtel, Jr. Foundation and by the National Science Foundation under Grant No. 0952013 and Grant No. 0934931. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the S.D. Bechtel, Jr. Foundation or the National Science Foundation. This project has also been made possible with support of the National Marine Sanctuary Foundation. The STAR program is administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESaME) on behalf of the California State University (CSU).



URL: https://digitalcommons.calpoly.edu/star/147


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