College - Author 1

College of Engineering

Department - Author 1

Mechanical Engineering Department

Degree Name - Author 1

BS in Mechanical Engineering

College - Author 2

College of Engineering

Department - Author 2

Mechanical Engineering Department

Degree - Author 2

BS in Mechanical Engineering

College - Author 3

College of Engineering

Department - Author 3

Mechanical Engineering Department

Degree - Author 3

BS in Mechanical Engineering

College - Author 4

College of Engineering

Department - Author 4

Mechanical Engineering Department

Degree - Author 4

BS in Mechanical Engineering

Date

5-2022

Primary Advisor

Sarah Harding, College of Engineering, Mechanical Engineering Department

Abstract/Summary

Cell-free protein synthesis (CFPS) is a growing field of study that involves the production of proteins without the need for living cells to be a part of the reaction. When conducting CFPS reactions, bioreactors are commonly used to control and optimize environmental factors such that high yields can be obtained. Most commercially available bioreactors are not suited for use in research and development or in smaller laboratories as they are expensive and typically require 100s of milliliters of fluid volume. Additionally, many of these systems are complex to interface with and require hours of training to use properly. In this report, we will detail our design, manufacturing, and testing of a small-scale, low-cost bioreactor capable of oxygenating CFPS reactions. The results of this testing indicate that we were successful in developing such a device that consistently and accurately maintains the dissolved oxygen levels in the fluid. This achievement indicates that our bioreactor might be the optimal tool for conducting additional research into CFPS in typical lab settings.

Available for download on Saturday, June 03, 2023

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