College - Author 1
College of Engineering
Department - Author 1
Mechanical Engineering Department
Degree Name - Author 1
BS in Mechanical Engineering
College - Author 2
College of Engineering
Department - Author 2
Mechanical Engineering Department
Degree - Author 2
BS in Mechanical Engineering
College - Author 3
College of Engineering
Department - Author 3
Mechanical Engineering Department
Degree - Author 3
BS in Mechanical Engineering
College - Author 4
College of Engineering
Department - Author 4
Mechanical Engineering Department
Degree - Author 4
BS in Mechanical Engineering
Date
6-2020
Primary Advisor
Peter J. Schuster, College of Engineering, Mechanical Engineering Department
Abstract/Summary
The Inverted Fluorescence Microscope senior project team at Cal Poly, San Luis Obispo designed, assembled, and tested a proof-of-concept inverted fluorescence microscope for the university’s Microfabrication Laboratory. Administrators of the laboratory wished to use fluorescence for research and experiments involving cell growth and flow visualization on the micro-scale, and did not have the budget to purchase one of the costly commercially available options. The scope of this design challenge was to produce a low-cost inverted fluorescence microscope employing available optical components and additional readily sourced parts to expand the use of fluorescence microscopy accessible to undergraduate students in the Microfabrication Laboratory.
This document is an account of the final microscope design as well as the engineering design process, project management procedures, and timeline followed to produce a working design verification prototype. The final product successfully resolved images of microfluidic devices in brightfield mode with automated maneuverability in the X-Y plane. It is equipped with fluorescence capabilities, and will serve as a valuable, low-cost research tool and platform for future student projects.
URL: https://digitalcommons.calpoly.edu/mesp/536