Title

Cartilage growth and remodeling: modulation of balance between proteoglycan and collagen network in vitro with β-aminopropionitrile

Author Info

Anna Asanbaeva, University of California - San Diego
Koichi Masuda, Rush University
Eugene J-M.A. Thonar, Rush University
Stephen M. Klisch, California Polytechnic State University - San Luis ObispoFollow
Robert L. Sah, University of California - San Diego

Recommended Citation

Published in Osteoarthritis and Cartilage, Volume 16, Issue 1, January 1, 2008, pages 1-11.

Abstract of paper.

The definitive version is available at https://doi.org/10.1016/j.joca.2007.05.019.

Abstract

Objective

To examine the effect of β-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro.

Design

Immature bovine articular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or without BAPN. Variations in tissue size, accumulation of proteoglycan and collagen (COL), and tensile mechanical properties were assessed.

Results

The inclusion of serum resulted in expansive tissue growth, stimulation of proteoglycan and COL deposition, and a diminution of tensile integrity. Supplementation of medium with BAPN accentuated this phenotype in terms of a further increase in tissue size in explants from the superficial layer and further diminution of tensile integrity, without affecting the contents of proteoglycan and COL in explants from both the superficial and middle layers.

Conclusion

COL crosslinking is a major factor in modulating the phenotype of cartilage growth and the associated balance between proteoglycan content and integrity of the COL network.

Disciplines

Mechanical Engineering