Effects of varying doses of oral bisphenol A consumption on type 2 diabetes risk markers in healthy adults


In animals, oral consumption of bisphenol A (BPA) alters diabetes risk markers but studies in humans are lacking. The objective of this study was to determine the effects of varying doses of oral BPA consumption on diabetes risk markers. Eleven healthy, college students (21.0 ± 0.8 yrs; 24.2 ± 3.9 kg/m2) were randomized in a double-blinded, cross-over fashion separated by >1 week to oral consumption of Placebo (PL), deuterated BPA at 4 mg/kg-BW (BPA-4), and deuterated BPA at 50 mg/kg-BW (BPA-50). Total BPA, glucose, insulin, C-Peptide, and other risk markers were assessed at baseline, minutes 15, 30, 45, 60, and every 30 minutes for 2 hours in response to a glucose tolerance test and analyzed using a linear mixed model. Total BPA concentration max was 159 ng/mL for BPA-50 and 25 ng/mL for BPA-4. There was a significant condition x time interaction for total serum BPA such that total serum BPA increased more rapidly in BPA-50 than in BPA-4 and PL (P0.05). However, significant main effects for condition were observed for glucose such that BPA-50 was significantly lower than PL (P=0.036) and nearly significantly lower for BPA-4 vs. PL (P=0.056). Significant condition main effects were observed on other risk markers: insulin in BPA-50 was lower than BPA-4 (P=0.021) and C-Peptide in BPA-50 was lower than BPA-4 (P=0.003). An oral BPA consumption protocol is feasible and may have immediate effects on diabetes risk markers in humans.

General Description of Collection Methods:

Venous blood samples were collected in sterile syringes and transferred to vacutainers containing sodium fluoride and potassium oxalate for analysis of glucose, Edetic acid (EDTA) for analysis of insulin, C-Peptide, and Proinsulin, and serum for analysis of total BPA, 17-beta estradiol and triglycerides. All samples were refrigerated centrifuged (4° C) at (3,000 g) for 15 min, and plasma/serum was aliquoted into polystyrene tubes and stored at -80°C until analysis. Quantitative assessment of total BPA, insulin, C-Peptide, Proinsulin, and 17-beta estradiol were determined by competitive enzyme-linked immunosorbent assay (ELISA) kits.



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