Title

Design and Experimental Validation of Continuous DNA Separation using ITP-Based Microfluidic Device

Author(s) Information

Nora C. M. Goscinski, California Polytechnic State University, San Luis ObispoFollow
Mary E. Rosa, California Polytechnic State University, San Luis Obispo
Elizabeth K. Wheeler, California Polytechnic State University, San Luis ObispoFollow
Maxim Shusteff, California Polytechnic State University, San Luis ObispoFollow

College - Author 1

College of Science and Mathematics

Department - Author 1

Chemistry and Biochemistry Department

Degree Name - Author 1

BS in Biochemistry

Date

1-2012

Primary Advisor

Christina Bailey

Abstract/Summary

We have demonstrated a chip-based microfluidic device suitable for rapid and high-throughput DNA separation and purification for downstream analysis. The glass-based device incorporates a transverse free-flow isotachophoresis (tFF-ITP) that overcomes the volume limitations of CE approaches, and allows continuous sample processing in contrast to batch-mode solid-phase extraction (SPE). The device is able to focus DNA at flow rates up to 100 uL/min, and sample conductivity up to 2 mS/cm. Downstream of the chip, 30-40% of DNA from the input sample is recovered as a result of ITP focusing, and preliminary results indicate that this design is able to purify DNA from contaminating species, particularly those that inhibit PCR.

URL: https://digitalcommons.calpoly.edu/chemsp/6