College - Author 1

College of Engineering

Department - Author 1

Biomedical Engineering Department

Degree Name - Author 1

BS in Biomedical Engineering

College - Author 2

College of Engineering

Department - Author 2

Biomedical Engineering Department

Degree - Author 2

BS in Biomedical Engineering

College - Author 3

College of Engineering

Department - Author 3

Biomedical Engineering Department

Degree - Author 3

BS in Biomedical Engineering



Primary Advisor

Christopher Heylman, College of Engineering, Biomedical Engineering Department

Additional Advisors

Andres Martinez, College of Science and Mathematics, Chemistry and Biochemistry Department


The following document describes major aspects of the project: Incorporating Antibodies Into Reagent Pencils For Lateral Flow Immunoassays. This project has been developed under the direction of Dr. Andres Martinez with the goal of creating a customizable lateral flow assay (LFA) kit consisting of antibody-specific reagent pencils and modifiable testing strips. Previous studies directed by Dr. Martinez have demonstrated effectiveness in producing a polymer-based enzyme matrix and using the matrix to deposit enzymes on paper test strips.

For the sake of time and resources, reagent pencils and LFA testing strips were constructed to detect the presence of human chorionic gonadotropin (hCG) and luteinizing hormone (LH) via liquid samples. The generic LFA test strip is modified with both primary and secondary reagent pencils. The primary pencils contain labeled antibodies for hCG and LH. The secondary pencil contains a universal capture antibody. A detailed design of the prototype models was developed for reagent pencil cores, generic LFA strips, and an acrylic strip case.

Each reagent pencil core was found to have a lifetime of about 450 tests and can operate in 0-80% relative humidity and 4-37.8℃. Currently, the reagent pencils have been proven effective at least 5 months after manufacturing. Additionally, hCG and LH reagent pencils produce positive test lines on the same universal lateral flow assay strip. Deliverables in this report include a conceptual design report, prototype development and manufacturing plans, documentation and analysis of all testing procedures, a complete budget, a final project report, and a functional LFA kit containing test strips and reagent pencils.

Available for download on Tuesday, March 14, 2028

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