College - Author 1
College of Engineering
Department - Author 1
Biomedical Engineering Department
Degree Name - Author 1
BS in Biomedical Engineering
College - Author 2
College of Engineering
Department - Author 2
Biomedical Engineering Department
Degree - Author 2
BS in Biomedical Engineering
College - Author 3
College of Engineering
Department - Author 3
Biomedical Engineering Department
Degree - Author 3
BS in Biomedical Engineering
Date
3-2020
Primary Advisor
Ben Hawkins, College of Engineering, Biomedical Engineering Department
Additional Advisors
Christopher Heylman, College of Engineering, Biomedical Engineering Department; Michael Whitt, College of Engineering, Biomedical Engineering Department
Abstract/Summary
This project report provides detailed information on designing and building a DIY Incubator. The device is indicated for growing and maintaining BSL-1 mammalian or bacterial cell culture with a temperature control in the range of 25-45°C ± 1°C. The device is made fully programmable via an Arduino, and is structurally designed to fit two T75 cell culture flasks at a time. The device is able to return to a specified temperature set point within 10 minutes and maintains 5% CO2 partial pressure conditions. The incubator has a small physical footprint, with an overall dimension of 22in x 16in x 15.5in (LxWxH). The system is intended to be used in a university laboratory by students and researchers. After manufacturing and testing the system, all specifications were met except for the specification of 5% ± 0.25% CO2 control. Our CO2 system set up has promising usability, but requires revisions and additional testing to meet its desired specification. This report will go into detail on the results of the tests that passed and why its specification was not met during testing, as well as future recommendations for this system. We are confident this device will have useful implications for cell cultivation research labs on campus.
URL: https://digitalcommons.calpoly.edu/bmedsp/133