Recommended Citation
Published in Biomedical Optics Express, Volume 7, Issue 5, January 1, 2016, pages 1690-1701.
The definitive version is available at https://doi.org/https://doi.org/10.1364/BOE.7.001690.
Abstract
In this work we demonstrate a label-free optical imaging technique to assess metabolic status and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes by two-photon fluorescence lifetime imaging of endogenous fluorophores. Our results show the sensitivity of this method to detect shifts in metabolism and oxidative stress in the cardiomyocytes upon pathological stimuli of hypoxia and cardiotoxic drugs. This non-invasive imaging technique could prove beneficial for drug development and screening, especially for in vitro cardiac models created from stem cell-derived cardiomyocytes and to study the pathogenesis of cardiac diseases and therapy.
Disciplines
Biomedical Engineering and Bioengineering
Copyright
© 2016 Optical Society of America
Number of Pages
12
Publisher statement
© 2016 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
URL: https://digitalcommons.calpoly.edu/bmed_fac/90