Completion Date
1-2026
Advisor(s)
Trevor Cardinal, Philip Costanzo
Abstract
Human macrophage isolation was successful with a 10% yield. Isolations are being further refined using an autoMACS cell separator, providing students instructional training on industry-level equipment that is commonly used. Through the primary human myoblast and murine macrophage co-culture, a significant shift in phenotype was demonstrated. Macrophage morphology varies with polarization and was used to evaluate the impact of factors and myoblasts at polarizing macrophages. Macrophage morphology was measured using an Image J analysis that quantified the axial ratio of macrophages. Axial ratio was calculated using the major axis divided by minor axis. The results showed that all the pre-polarized M0, M1, and M2 RAW 264.7 murine macrophages after 48 hours of coculture demonstrated a higher axial ratio than the pre-polarized monocultured M0, M1, and M2 macrophages. In vitro coculture of RAW 264.7 macrophages with primary human myoblasts leads to the polarization of M0 macrophages to an M2 phenotype, reverses the polarization of M1 macrophages to an M2 phenotype, and enhances the polarization of pre-polarized M2 regenerative macrophages.
URL: https://digitalcommons.calpoly.edu/bkendowments/115
Copyright
This work is licensed under a Creative Commons Attribution 4.0 International License.