Title

Removal of PCR Inhibitors From Soil DNA by Chemical Flocculation

Author Info

Michael D. Braid, Ciphergen Biosystems, Inc.
Laura M. Daniels, University of California - Davis
Christopher L. Kitts, California Polytechnic State University - San Luis ObispoFollow

Recommended Citation

Postprint version. Published in Journal of Microbiological Methods, Volume 52, Issue 3, March 1, 2003, pages 389-393.

The definitive version is available at https://doi.org/10.1016/S0167-7012(02)00210-5.

Abstract

Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH4(SO4)2 during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.

Disciplines

Biology

Copyright

2003 Elsevier, Inc.