"Comparison of the Effects of Growth Hormone on MAC-T Cells and Primary" by Lisa McDonnell, Tasha Johnson et al.
 

Abstract

The ability of growth hormone (GH) to increase milk yield through somatomedins is well characterized,however recent studies utilizing mammary epithelial cell (MEC) lines have indicated a possible direct effecton MEC. In the mammary alveolar cell-T (MAC-T) line, relative abundance of mRNA for two milk protein genes, α-lactalbumin and αS1-casein, and GH receptor was increased by GH. MAC-T cells were compared tobovine mammary tissue explants and primary MEC isolated from raw milk through culture in classicallactation medium (dexamethasone, insulin, prolactin) with 0 or 10 ng/mL GH. Explant and primary cellmRNA abundance showed a similar pattern to MAC-T indicating that the effects observed in cell lines maybe relevant in vivo. To begin to understand the nature of the effects of GH in MEC, global protein expressionin MAC-T cells was analyzed by 2-D gel electrophoresis, finding the abundance of 40 proteins altered byinclusion of 10ng/mL GH. Proteins were identified using MALDI-TOF with tandem MS and include enzymesinvolved in glycolysis, lipogenesis, protein processing and transport.

Disciplines

Animal Sciences

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URL: https://digitalcommons.calpoly.edu/asci_fac/22