Recommended Citation
Published in The Journal of Histochemistry and Cytochemistry, Volume 31, Issue 7, January 1, 1983, pages 956-959.
Article URL: http://www.jhc.org/cgi/reprint/31/7/956
NOTE: At the time of publication, the author Susan C. Opava-Stitzer was affiliated with the University of Puerto Rico School of Medicine - San Juan, Puerto Rico. Currently, April 2008, she is Dean of Research and Graduate Programs at California Polytechnic State University - San Luis Obispo.
Abstract
Although several studies have indicated that antidiuretic hormone (ADH) enhances the phagocytic function of the reticuloendothelial system (RES) in shock syndromes, it remains unknown what influence ADH exerts upon the individual phagocytic components of this system. The present investigation was designed to evaluate the effects of endogenous ADH on the phagocytic activity of peritoneal macrophage cells. As a phagocytic stimuli, fluorescent methacrylate microbeads were injected intraperitoneally into Brattleboro (ADH deficient) and normal Long Evans rats in the presence and absence of exogenous ADH. Peritoneal cells were harvested 19-22 hr after the administration of the microbeads and the percent phagocytosis was determined in macrophage cells using a fluorescence-activated cell sorter (FACS II). Our results indicate that the percentage of peritoneal macrophages ingesting the fluorescent methacrylate microbeads was significantly reduced in the absence of ADH (Brattleboro rats: 5.4 ± 0.6% versus Long Evans rats: 16.8 ± 2.3%; p < 0.001). In addition, our data demonstrate that exogenous administration of ADH significantly enhanced macrophage phagocytosis in Brattleboro (14.7 ± 2.2%) and normal Long Evans (49.6 ± 4.5%) rats. These data suggest, for the first time, that endogenous ADH might play a modulatory role in the phagocytic activity of a specific component of the RES, namely, the macrophage cell.
Disciplines
Physiology
URL: http://digitalcommons.calpoly.edu/rgp_dean/2