October 1, 2017.
The purpose of this research is to synthesize a crossinker that is MS-labile and that produces fragments under MS (mass spectrometry) conditions that allows masses of the peptides determined by the name of LXR-SEB (Labile Crosslinker Reagent-Succinic Ethanolamine Biotin). The samples were prepared in a wet lab at the Institute for Systems Biology, tested using crosslinking MS methods, and finally purified using FPLC (Fast Protein Liquid Chromatography). The results were further analyzed by myself and my mentor, Dr. Isil Hamdemir. The final results showed that we did successfully obtain pure tEB and LXR-Amine, however, SEB was not obtained in the time allotted. When successfully formed and obtained in adequate amounts, this crosslinker will ultimately assist in the future of healthcare by allowing scientists to not only understand more about PPIs (protein-protein interactions) but also about what occurs inside a cell when it becomes diseased.
Institute for Systems Biology (ISB)
This material is based upon work supported by the National Science Foundation through the Robert Noyce Teacher Scholarship Program under Grant # 1340110. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The research was also made possible by the California State University STEM Teacher and Researcher Program, in partnership with Chevron (www.chevron.com), the National Marine Sanctuary Foundation (www.marinesanctuary.org) and Institute for Systems Biology.