October 1, 2017.
Curcumin (Curcuma longa) is a plant-based polyphenol known to have several medicinal properties. Although several promising effects of using curcumin in clinical trials have been observed, its overall medicinal qualities are still limited due to low bioavailability. In order to increase the bioavailability, we are embedding curcumin within Nano-Lipid Particles (both curcumin telodendrimer discs and curcumin tNLPs). Telodendrimer nanolipoprotein particles (tNLPs) are discoidal self-assemblies containing lipids and apolipoproteins which can be used as a vehicle to carry proteins and other small molecules to the cell. Telodendrimer NLPs have been used to increase the bioavailability of drugs, and provide an ideal platform to increase curcumin bioavailability. The generation of tNLPs can be accomplished using several methods; such as cellfree assembly and in-vitro assembly. Curcumin telodendrimer discs (curcumin telo-discs) are a nano-lipid mixture of lipids, curcumin, and telodendrimer that acts as the basis for the curcumin tNLP reaction. Using the curcumin telo-disc as the starting additive, we demonstrate that we can purify properly formed curcumin tNLPs via affinity columns and size-exclusion chromatography (SEC). Here, we show that with two separate methods: a cell-free expressed method and in-vitro assembly, we can demonstrate that curcumin.
Lawrence Livermore National Laboratory (LLNL)
This material is based upon work supported by the National Science Foundation through the Robert Noyce Teacher Scholarship Program under Grant # 1418852. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The research was also made possible by the California State University STEM Teacher and Researcher Program, in partnership with Chevron (www.chevron.com), the National Marine Sanctuary Foundation (www.marinesanctuary.org) and Lawrence Livermore National Laboratory.