September 1, 2016.
Cells contain many genes that encode proteins which dictate the cell function. The thousands of genes expressed in a particular cell determine what the cell can do. When a cell is damaged, there are powerful mechanisms to repair damaged genes. Here, we investigated different cell lines that have been exposed to ionizing radiation (IR). To understand the effect of genes to different doses of IR and their correlations to up-regulated and down-regulated genes, a biomarker study has been performed on the following different cell types: In vivo lymphocytes from Metaiodobenzylguanidine (MIBG) isotopic radiation treated neuroblastoma patients and in vitro lymphoblastoid as well as fibroblast cells externally exposed to radiation. Results of the study concluded that qRT PCR analysis can be used to illustrate different trends between irradiated samples. The externally radiated in vitro lymphoblastoid and fibroblast samples showed positive fold change differences for the majority of transcripts studied. In addition, the fibroblast cells showed the highest expression of transcripts compared to the other two studies. This knowledge can be applied to accidental radiation exposures and other biodefense mechanisms. It allows us to understand the process of molecular changes, predict the outcome, and monitor radiation progression.
Lawrence Livermore National Laboratory (LLNL)
This material is based upon work supported by the National Science Foundation through the Robert Noyce Teacher Scholarship Program under grant# 1240040. Any opinions, finding, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The research was made possible by the California State University STEM Teacher Researcher Program in partnership with Lawrence Livermore National Laboratory. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore NaFonal Laboratory under Contract DE-AC52-07NA27344.² IM #830119