Postprint version. Published in Australian Journal of Experimental Agriculture, Volume 30, Issue 4, January 1, 1990, pages 557-563.
NOTE: At the time of publication, the author G.S.P. Ritchie was not yet affiliated with Cal Poly.
The definitive version is available at https://doi.org/10.1071/EA9900557.
Navy beans (Phaseolus vulgaris cv. Gallaroy) were grown with 7 rates of zinc (Zn) in a Zn-deficient gravelly sandy loam in a glasshouse experiment. The plant shoots were harvested 31 days after sowing and the Zn concentration in each of 4 plant parts (YL, young leaf; YOL, young open leaf; YFEL, youngest fully expanded leaf; and whole shoots) was related to the fresh weight of the shoots. The critical Zn concentrations (mgtkg) in the plant parts determined by the 2 intersecting straight lines model were 21.1 for YL (r2 = 0.66), 17.1 for YOL (r2 = 0.83), 10.6 for YFEL (r2 = 0.91) and 12.5 for the whole tops (r2 = 0.88). The YFEL was selected as an appropriate diagnostic tissue because it is readily identifiable in the field and had the highest r2 with fresh weight.
In a second glasshouse experiment, the critical Zn concentration in the YFEL and 5 soil tests were evaluated for their ability to predict the Zn status of navy beans. There were 13 soils from sands to clays with a wide range of chemical properties. The soil tests were 0.1 mol/L HCl, DTPA, EDTA, dilute CaCl2 and soil solution Zn. The concentration of Zn in the YFEL correctly predicted Zn deficiency or adequacy in about 77% of samples. The results from both experiments showed that a critical Zn concentration of 10-11 mg/kg in the YFEL can be used to diagnose the Zn status of Gallaroy navy beans.
It was not possible to recommend a single soil test for prediction of the relative yield of navy beans. A combination of quantity (HCl, EDTA, DTPA) and intensity (soil solution, 0.002 mol/L CaCl2, 0.01 mol/L CaCl2) parameters were able to explain most of the variation in the Zn concentration of the YFEL, a more sensitive measure of nutrient availability than relative yield. EDTA-Zn in combination with 0.01 mol/L CaCl2-Zn explained 90% of the variation in the Zn concentration in the YFEL, while HCl- or DTPA-Zn and 0.01 mol/L CaCl2 explained about 80% of the variation. As soil solution Zn was significantly correlated with 0.002 and 0.01 mol/L CaCl2-Zn (r = 0.75, P2-Zn may be used as a more convenient measure of Zn intensity than soil solution Zn.
Food Science | Nutrition