Title

β-Casein–phospholipid monolayers as model systems to understand lipid–protein interactions in the milk fat globule membrane

Author Info

Sophie Gallier, University of Otago
Derek E. Gragson, California Polytechnic State University, San Luis ObispoFollow
Rafael Jiménez-Flores, California Polytechnic State University - San Luis ObispoFollow
David W. Everett, University of Otago

Recommended Citation

Postprint version. Published in International Dairy Journal, Volume 22, Issue 1, January 1, 2012, pages 58-65.

The definitive version is available at https://doi.org/10.1016/j.idairyj.2011.08.007.

Abstract

Phospholipid–protein monolayer films were studied as model systems to mimic the structure of the native bovine milk fat globule membrane (MFGM) and to understand lipid–protein interactions at the surface of the globule. Phospholipids extracted from bovine raw milk, raw cream, processed milk and buttermilk powder were spread onto the air–water interface of a Langmuir trough, β-casein was then added to the sub-phase, and Langmuir–Blodgett films were studied by epifluorescence microscopy and atomic force microscopy. In all films, β-casein was responsible for clustering of the sphingomyelin- and cholesterol-rich microdomains into larger platforms. This suggests that the same phenomenon may happen at the surface of the milk fat globule, where specific MFGM proteins may cause aggregation of microdomains.

Disciplines

Biochemistry | Chemistry

Copyright

2012 Elsevier