Department

Biomedical and General Engineering Department

Degree Name

BS in Biomedical Engineering

Date

6-2012

Advisor(s)

Trevor Cardinal

Abstract/Summary

Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling and repair. For example, this approach can be used to examine the relationship between osteocyte apoptosis and angiogenesis in bone tissue, with applications to ischemic conditions and osteoporosis.

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