Bisphenols (BPA, BPS, BPF) are synthetic organic chemicals with endocrine disrupting properties that are routinely used in the production of plastics used for lining food and beverage containers as well as other products commonly used by consumers. Bisphenol exposure, widespread in the US with 93% of the population having detectable urine levels, is known to have negative health effects related to weight gain, obesity, cardiovascular disease and other chronic illnesses. Surprisingly, few studies have experimentally tested a randomized intervention to reduce bisphenol exposure. This study, ancillary to an ongoing clinical trial examining the effects of a weight loss intervention on disease risk markers and reoccurrence of gestational diabetes, examined whether the 4-month standard behavioral weight loss intervention reduces urine bisphenol exposure. Thirty women with overweight or obesity were recruited and randomized to a weight loss intervention or control group. We hypothesized that weight loss and reduced urinary bisphenol (BPA, BPS) levels would be correlated at 4 months. We used a competitive Enzyme-Linked Immunosorbent Assay (cELISA ) kit ran in triplicate to assess urinary BPA levels. The cELISA was analyzed with a Multiskan FC microplate photometer and concentration values were determined using GraphPad Prism 8 software by using a standard curve. A repeated measure analysis of variance (RMANOVA) was then used to determine differences in urine bisphenol concentrations. This pilot study revealed no association between reduced urinary bisphenol (BPA, BPS) levels and weight loss (P > 0.05). Future studies with larger sample sizes and increased magnitude of weight loss are needed to further evaluate the relationship between weight loss and reduction in bisphenol exposure.
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