The goal of this research project is to develop a simple and quantitative chronometric glucose test on printed microfluidic devices using polymer bound boronic acid (PBBA), which selectively binds to glucose, instead of enzymatic reactions. PBBA promises to enable cheaper medical diagnostics without requiring the expensive and often complex instrumentation that is normally utilized in medical diagnostics. PBBA should also increase the shelf life of glucose tests as the material is not as susceptible to degradation as the enzymatic reagents that are currently in use. The final device will be optimized to measure glucose concentrations in the range of 0.5 to 10 mM using 20 to 40 μL of a liquid sample in a time frame under 30 minutes. The results gained from this project will serve as a foundation for further research into the use of nonenzymatic polymer-based assays in printed microfluidic devices.
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