Postprint version. Published in Applied and Environmental Microbiology, Volume 71, Issue 8, August 1, 2005, pages 4925-4929.
The definitive version is available at https://doi.org/10.1128/AEM.71.8.4925-4929.2005.
Two genes, bshA and bshB, encoding bile salt hydrolase enzymes (EC 18.104.22.168) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes.