Title

Characterization of the Differentiation and Leptin Secretion Profile of Adult Stem Cells on Patterned Polylactide Films

Author Info

Aditya Chaubey, Clemson University
Kevin J. Ross, University of North Carolina at Chapel HillFollow
Malcolm R. Leadbetter, University of North Carolina at Chapel Hill
Cheryl T. Gomillion, Clemson University
Karen J.L. Burg, Clemson University

Recommended Citation

Postprint version. Published in Journal of Biomaterials Science, Polymer Edition, Volume 20, Issue 7-8, April 2, 2012, pages 1163-1177.

NOTE: At the time of publication, the author Kevin Ross was not yet affiliated with Cal Poly.

The definitive version is available at https://doi.org/10.1163/156856209X444484.

Abstract

Several issues need to be better understood before breast tissue engineering becomes a clinically viable option. One of the most important aspects is the interaction between cells and the microtopography of the implant surface. The aim of this study was to evaluate the efficacy of D1 cells, multipotent mouse bone marrow stromal precursors, in differentiating to adipocytes and to characterize their metabolic activity (lactic acid released and glucose consumed), leptin secretion and lipid production when cultured on patterned poly(L-lactide) (PLLA) films. It was determined that, by appropriate stimulation, the D1 cells displayed morphological characteristics of adipocytes and produced lipid. The results showed that a patterned surface did affect the rate of lipid production. Polynomial models were proposed to predict the amount of leptin secreted by the cells over a period of time.

Disciplines

Statistics and Probability

Copyright

2009 Taylor & Francis.

Publisher statement

This is an electronic version of an article published in Journal of Biomaterials Science, Polymer Edition.