Abstract

Electrolyte composition, concentration, pH, ionic strength, and temperature have long been known to play a role in the interface of the electrode-electrolyte. However, detailed studies are not sufficient. Cyclic voltammetry (CV) is a widely used electrochemical technique for obtaining qualitative information in electroanalysis. CV is often the first experiment performed in an electrode surface in an electrochemical study. It measures the number of electrons transferred and intermediates formed during oxidation-reduction (redox) reactions by observing its current, intensity and position of peak potential with applied voltage. This is accomplished by using a three-electrode system including working electrode (WE), counter electrode (CE) and reference electrode (RE). In this work, our system consists of gold (Au) We, platinum (PT) CE and silver/silver chloride (Ag/AgCl) RE. The potential relative to the reference electrode is forward- and reverse-scanned at the working electrode while the current flows through the counter electrode is observed in a solution. In this study, we investigate systematically the redox potential of the different analytes under various conditions in concentration and pH. Therefore, this study can be referenced for studies in exploring a variety range of biological samples, cells, tissues, lipids, and proteins on surfaces.

Disciplines

Biotechnology | Laboratory and Basic Science Research

Mentor

Xiao-Ying Yu

Lab site

Pacific Northwest National Laboratory (PNNL)

Funding Acknowledgement

This material is based upon work supported by the Howard Hughes Medical Institute (HHMI). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of HHMI. This work was administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESAME) and the Fresno State Science and Mathematics Education Center (SMEC) on behalf of the California State University.

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URL: http://digitalcommons.calpoly.edu/star/267

 

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