Abstract

Mammalian cells are known to express genes that are associated with repairing damaged DNA. The transcript CDKN1A is one of several cell cycle regulator genes expressed in response to cell damage by ionizing radiation (IR). In this study, male and female lymphocytes; previously exposed ex vivo to IR, were used to demonstrate linear gene expression responses that may vary between genders. We used qRT-PCR to generate response curves for CDKN1A. No differences were identified for the endogenous control gene GAPDH. CDKN1A expression demonstrated average fold changes well above three fold for three of the four healthy patient donors at 24 hours after 2, 3, and 4 Gy exposures. Doses 2 and 3 Gy were significantly up-regulated at 24 hours. No significant difference was seen between genders for CDKN1A. Our data confirms that genes involved in DNA repair, cell cycle arrest, and apoptosis can be used as biomarkers of exposure to IR. Because of growing concern of IR exposure through different mechanisms; either by nuclear catastrophe or medical radiation, gene expression analysis is a promising method for identification and estimation of IR exposure.

Disciplines

Biophysics | Biotechnology | Genetics

Mentor

Matthew Coleman

Lab site

Lawrence Livermore National Laboratory (LLNL)

Funding Acknowledgement

This material is based upon work supported by the S.D. Bechtel, Jr. Foundation and by the National Science Foundation under Grant No. 0952013. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the S.D. Bechtel, Jr. Foundation or the National Science Foundation. This project has also been made possible with support of the National Marine Sanctuary Foundation. The STAR program is administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESaME) on behalf of the California State University (CSU)., This material is based upon work supported by the S.D. Bechtel, Jr. Foundation and by the National Science Foundation under Grant No. 0952013 and Grant No. 0833353. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the S.D. Bechtel, Jr. Foundation or the National Science Foundation. This project has also been made possible with support of the National Marine Sanctuary Foundation. The STAR program is administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESaME) on behalf of the California State University (CSU)., This material is based upon work supported by the Howard Hughes Medical Institute (HHMI). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of HHMI. This work was administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESAME) and the Fresno State Science and Mathematics Education Center (SMEC) on behalf of the California State University.

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URL: http://digitalcommons.calpoly.edu/star/232

 

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