Abstract

Bead homogenization is a mechanical method utilizing microscopic glass beads for lysing Bacillus spore cells to extract DNA. Once DNA is extracted, real-time PCR may be run on sample DNA to identify species or compare relative amount of start DNA within samples. This experiment analyzes the efficiency of a machines, containers, and container positioning used in mechanical spore lysing and DNA extraction. Results show spore samples lysed in single tube machines produced more DNA material than high volume multi-sample machines and containers

Mentor

Bill Hoppes

Lab site

Lawrence Livermore National Laboratory (LLNL)

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URL: http://digitalcommons.calpoly.edu/star/23

 

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