January 1, 2010.
Bead homogenization is a mechanical method utilizing microscopic glass beads for lysing Bacillus spore cells to extract DNA. Once DNA is extracted, real-time PCR may be run on sample DNA to identify species or compare relative amount of start DNA within samples. This experiment analyzes the efficiency of a machines, containers, and container positioning used in mechanical spore lysing and DNA extraction. Results show spore samples lysed in single tube machines produced more DNA material than high volume multi-sample machines and containers
Lawrence Livermore National Laboratory (LLNL)