Abstract

The research is testing the limits of detection and multiplexing with the use of three pathogens with the machine created from Luminex called Magpix. The pathogens are Salmonella enterica, Listeria monocytogenes, and Escherichia Coli O157. The gene that is being focused on for each pathogen is invA for Salmonella, hlyA for listeria, and hlyA for Escherichia Coli. In this research, we started with prepared probes that were attached to the magnetic microsphere beads. Then we extracted and amplify the DNA. Furthermore, the probes were combined to the amplified DNA and used this in Magpix which gave a corresponding MFI value.

Disciplines

Educational Leadership | Science and Mathematics Education

Mentor

Sonam Sahni

Lab site

California State University, Fresno (CSU Fresno)

Funding Acknowledgement

This material is based upon work supported by the S.D. Bechtel, Jr. Foundation and by the National Science Foundation under Grant No. 0952013. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the S.D. Bechtel, Jr. Foundation or the National Science Foundation. This project has also been made possible with support of the National Marine Sanctuary Foundation. The STAR program is administered by the Cal Poly Center for Excellence in Science and Mathematics Education (CESaME) on behalf of the California State University (CSU).

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URL: http://digitalcommons.calpoly.edu/star/167

 

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