N utilization, including plant dry weight (DW) production, total N and nitrate N (NO3-N) content accumulation, N utilization efficiency (NUE), root absorption efficiency (RAE), reduced N accumulation, and nitrate assimilation capacity (NAC) were determined for creeping bentgrass cultivars grown in hydroponic culture. Possible mechanisms affecting N utilization including nitrate reductase activity (NRA) and root morphology were also examined. Four cultivars, selected from an initial screening of creeping bentgrasses were grown under both low and high levels of N in a flowing solution culture system. The relationship between NUE and plant growth for two different creeping bentgrass cultivars was investigated through tissue culture. The results indicate a genotypic variation in N utilization and absorption. The NAC was not the primary factor involved in genotypic differences in NUE. However, NRA was probably one of the mechanisms for the regulation of NUE. N utilization was affected by the level of N supplied level and environmental conditions. Plants grown under low levels of N had longer roots compared to plants grown under medium or high levels of N for either cultivar. The results suggest that root formation was probably one of the mechanisms for regulating the nitrate utilization in creeping bentgrass.


Agronomy and Crop Sciences



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