Date

3-2013

Degree Name

BS in Biomedical Engineering

Department

Biomedical and General Engineering Department

Advisor(s)

David Clague

Abstract

Microfluidic devices are small portable diagnostic chips that allow for analysis of a biologic sample at relatively low cost. This makes them ideal for settings where a hospital is unavailable. The microfluidic pretreatment isoelectric focusing DC-field assisted H-filter is a device that allows for the separation of differently charged particles in a biologic sample. It does this by employing the concepts of diffusion, electrophoresis, and isoelectric focusing. This is perfect for isolation of a desired analyte or separation of waste from a sample in order to achieve better diagnostic results. The device was first modeled mathematically and visually using Comsol. The device was then manufactured using laser cut acrylic, bibulous paper, acrylic cement, tygon tubing, and silicon adhesive. Finally the device was tested using charged fluorescent microspheres. There were many problems with implementation of the device and it was eventually found to not meet the specifications required.