"16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Ba" by Christopher W. Kaplan, Johanna C. Astaire et al.

Biological Sciences

Title

16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Bacterial Communities in Feces of Rats Fed Lactobacillus acidophilus NCFM

Author Info

Christopher W. Kaplan, California Polytechnic State University - San Luis Obispo
Johanna C. Astaire, California Polytechnic State University - San Luis Obispo
M. E. Sanders, California Polytechnic State University - San Luis Obispo
Bandaru S. Reddy, American Health Foundation, Valhalla, NY
Christopher L. Kitts, California Polytechnic State University - San Luis Obispo

Recommended Citation

Postprint version. Published in Applied and Environmental Microbiology, Volume 67, Issue 4, May 1, 2001, pages 1935-1939. Copyright © 2001 American Society for Microbiology. All Rights Reserved. The definitive version can be found online at http://dx.doi.org/10.1128/AEM.67.4.1935-1939.2001.

Abstract

16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics of Lactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence of L. johnsonii was confirmed by isolation from feces.